As a pharmaceutical researcher specializing in formulation, I have developed extensive expertise in the design and characterization of lipids formulations for mRNA/siRNA delivery. My research focuses on utilizing the zebrafish model to explore the targeting mechanism of peptide-modified liposomes. My work also examines the impact of shielding lipids, polysarcosine, on the biodistribution and transfection efficacy of lipoplexes. Additionally, I investigate how different preparation methods and the compositions of cationic and ionizable lipids influence lipoplexes efficacy.

d.bi@lacdr.leidenuniv.nl

Leiden Academic Centre for Drug Research
LACDR/Drug Delivery Technology
Einsteinweg 55
2333 CC Leiden

Investigating lipid formulations for efficient RNA delivery using zebrafish models

2019 - 2023
D. Bi, et.al. Deconvolving Passive and Active Targeting of Liposomes Bearing LDL Receptor Binding Peptides Using the Zebrafish Embryo Model. Small, 2024, 2310781. https://doi.org/10.1002/smll.202310781 

D. Bi, et.al, Polysarcosine-based lipid formulations for intracranial delivery of mRNA. Journal of Controlled Release, 2023, 356, 1-13. 10.1016/j.jconrel.2023.02.021

Y.He, D. Bi, (co-first author) et.al. Development of a Combined Lipid-Based Nanoparticle Formulation for Enhanced siRNA Delivery to Vascular Endothelial Cells, Pharmaceutics, 2022, 14(10), 2086. 10.3390/pharmaceutics14102086

D. Bi, et.al. On the Influence of Fabrication Methods and Materials for mRNA-LNP Production: From Size and Morphology to Internal Structure and mRNA Delivery Performance in vitro and in vivo, Adv. Healthcare Mater. 2024, 2401252. https://doi.org/10.1002/adhm.202401252